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iCas
(Plasmid #84232)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84232 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    GeneArt CRISPR Nuclease vector with OFP reporter
  • Backbone size w/o insert (bp) 5081
  • Vector type
    Mammalian Expression
  • Selectable markers
    OFP expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    Inducible Cas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    7969
  • Promoter CMV
  • Tags / Fusion Proteins
    • ERT2-ERT2 (N terminal on insert)
    • ERT2-ERT2 (C terminal on insert)
    • 2A-OFP co-expression (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI/SfoI (unknown if destroyed)
  • 3′ cloning site EcoRI/XbaI (unknown if destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The four copies of ERT2 are identical in sequence and may recombine occasionally. Hence, it best to use competent cells like STBL3 or NEB Stable to propagate the plasmid and to also check the plasmid size on an agarose gel.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iCas was a gift from Meng How Tan (Addgene plasmid # 84232 ; http://n2t.net/addgene:84232 ; RRID:Addgene_84232)
  • For your References section:

    A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing. Liu KI, Ramli MN, Woo CW, Wang Y, Zhao T, Zhang X, Yim GR, Chong BY, Gowher A, Chua MZ, Jung J, Lee JH, Tan MH. Nat Chem Biol. 2016 Sep 12. doi: 10.1038/nchembio.2179. 10.1038/nchembio.2179 PubMed 27618190