Fuw-dCas9-Dnmt3a_IM
(Plasmid #84478)

• Purpose: Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)
• Depositing Lab: Rudolf Jaenisch
• Publication: Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. doi: 10.1016/j.cell.2016.08.056.

Backbone

• Vector backbone: FUW
• Backbone size w/o insert (bp): 9207
• Total vector size (bp): 14313
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin ; Resistance marker is outside the LTRs and will not be packaged into virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9-Dnmt3a_IM
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 5106

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: aactatgcgctcggg
• 3′ sequencing primer: taaagcagcgtatcc

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry