Addgene - pCMV-VSV-G Plasmid Data

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Plasmid 8454: pCMV-VSV-G

Gene/insert name:		none
Insert size (bp):		Unknown
Vector backbone:		na (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		6363
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Bob Weinberg
Additional Documents:		Weinberg-viral (PDF)
Terms and Licenses:		MTA

Comments: VSVG envelope protein, for use with lentiviral and MuLV vectors. The 293T cell line can be obtained from the Weinberg lab or GenHunter (http://genhunter.com/products/aptag-3/index.html).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

163 - 450

CMV_immearly_promoter

84 - 660

5_LTR

148 - 974

CMV_fwd_primer

617 - 637

CMV_promoter

618 - 687

pCEP_fwd_primer

661 - 680

LNCX_primer

663 - 687

CMV2_promoter

630 - 749

bGlob_int

778 - 1350

Orf frame 1

1276 - 2955

vsv-G

1420 - 2955

rb_glob_PA_terminator

2974 - 3500

M13_pUC_rev_primer

3567 - 3545

lac_promoter

3610 - 3581

pBR322_origin

4538 - 3919

Orf frame 1

5553 - 4693

Ampicillin

5553 - 4693

AmpR_promoter

5623 - 5595

f1_origin

6122 - 5816

lacZ_a

6295 - 6140

M13_pUC_fwd_primer

6266 - 6288

M13_forward20_primer

6281 - 6297

T7_promoter

6307 - 6325

Unique restriction sites

SpeI		97
SalI		753
ApaI		1309
StuI		2119
PstI		2208
AgeI		2401
KpnI		2432
BclI		2504
BstBI		2681
MscI		3022
BglII		3051
SacII		6345
NotI		6351
XbaI		6358

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA et al. (RNA 2003 Apr;9(4):493-501. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 8454" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.