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PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP
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Depositing Lab
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Sequence Information
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Sequences (3) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUbC lentivirus expression vector (2nd generation)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
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Alt nameTIR1, IRES, scFv-sfGFP
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SpeciesSynthetic; Oryza Sativa
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Tag
/ Fusion Protein
- myc tag (9x) (C terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUbC-OsTIR1-myc-IRES-scFv-sfGFP was a gift from Robert Singer (Addgene plasmid # 84563 ; http://n2t.net/addgene:84563 ; RRID:Addgene_84563) -
For your References section:
Translation dynamics of single mRNAs in live cells and neurons. Wu B, Eliscovich C, Yoon YJ, Singer RH. Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084. Epub 2016 May 5. 10.1126/science.aaf1084 PubMed 27313041
