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pUbC-nls-ha-stdMCP-stdGFP
(Plasmid #98916)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98916 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUbC
  • Backbone size w/o insert (bp) 6250
  • Total vector size (bp) 8536
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    stdMCP-stdGFP
  • Species
    Synthetic
  • Insert Size (bp)
    2283
  • Promoter UbC
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer cgc cga tga tta tat aag ga (UbC)
  • 3′ sequencing primer cat aaa gag aca gca acc ag (Wpre)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUbC-nls-ha-stdMCP-stdGFP was a gift from Robert Singer (Addgene plasmid # 98916 ; http://n2t.net/addgene:98916 ; RRID:Addgene_98916)
  • For your References section:

    Synonymous modification results in high-fidelity gene expression of repetitive protein and nucleotide sequences. Wu B, Miskolci V, Sato H, Tutucci E, Kenworthy CA, Donnelly SK, Yoon YJ, Cox D, Singer RH, Hodgson L. Genes Dev. 2015 Apr 15;29(8):876-86. doi: 10.1101/gad.259358.115. Epub 2015 Apr 15. 10.1101/gad.259358.115 PubMed 25877922