Addgene - HA Raptor mutant 7 Plasmid Data

Plasmid 8475: HA Raptor mutant 7

Gene/insert name:
Raptor
Species:
H. sapiens (human)
Entrez Gene:
RPTOR (KOG1, Mip1)
Fusion protein or tag:
ha
Terminal:
N terminal on backbone
Mutation:
738SLQN741-PAAA (S738P, L739A, Q740A, N741A)
Vector backbone:
prk5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4716
Cloning site 5':
sal
Site destroyed during cloning:
Unknown
Cloning site 3':
not
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
we cloned this gene
Sequence: View sequences (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al (Cell 2002 Jul 26;110(2):163-75. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8475" in your Materials and Methods section.