PurposeFor Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85008||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
- Backbone size w/o insert (bp) 8187
- Total vector size (bp) 10264
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesAequorea victoria, Encephalomyocarditis virus, E coli, HSV
Insert Size (bp)2086
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer EBV_rev_primer (Common Sequencing Primers)
Terms and Licenses
Backbone plasmid (pK037) is available in Addgene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova) was a gift from Takuji Iwasato (Addgene plasmid # 85008 ; http://n2t.net/addgene:85008 ; RRID:Addgene_85008)
For your References section:Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045