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Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.
Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85006pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.
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85007pK036.TRE-Flpe-WPRE (Supernova)For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.
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85008pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.
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85038pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.
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85039pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova) For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.
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85040pK170.AAV-TRE-Cre-WPRE (Supernova)For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.
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85041pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova) This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.
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85179pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.
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89587pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPREFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.
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