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pK170.AAV-TRE-Cre-WPRE (Supernova)
(Plasmid #85040)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85040 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-TRE-MCS-WPRE
  • Backbone size w/o insert (bp) 3776
  • Total vector size (bp) 5510
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nlsCre-WPRE
  • Species
    SV40, P1 phage,Woodchuck hepatitis virus
  • Insert Size (bp)
    1734

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer GAGAACGTATGTCGAGGTAGGCGTGTAC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For the details of pAAV-TRE-MCS-WPRE, see Hayashi, Y. et al., Science 350, 957-961 (2016).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK170.AAV-TRE-Cre-WPRE (Supernova) was a gift from Takuji Iwasato (Addgene plasmid # 85040 ; http://n2t.net/addgene:85040 ; RRID:Addgene_85040)
  • For your References section:

    Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045