|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85681||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerEric Lander and David Sabatini (Addgene #71409)
- Backbone size (bp) 7000
Modifications to backboneReplaced sgRNA scaffold with sgRNA-(F+E)-combined optimized scaffold from Chen et al 2013 Cell.
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter hU6
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer LKO.1 5' GACTATCATATGCTTACCGT
- 3′ sequencing primer WPRE-R CATAGCGTAAAAGGAGCAACA (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:sgOpti was a gift from Eric Lander & David Sabatini (Addgene plasmid # 85681 ; http://n2t.net/addgene:85681 ; RRID:Addgene_85681)
For your References section:Systematic mapping of functional enhancer-promoter connections with CRISPR interference. Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, Kane M, Cleary B, Lander ES, Engreitz JM. Science. 2016 Sep 29. pii: aag2445. 10.1126/science.aag2445 PubMed 27708057