pCS2+ oDam_f_GFPoNLS
(Plasmid
#85819)
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PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4075
- Total vector size (bp) 5741
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Vector typeMammalian Expression, Bacterial Expression, Yeast Expression, Worm Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameoDam_f_GFPoNLS
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Alt nameoDamGFP
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SpeciesDam: E. coli; GFP: Aequorea viactoria
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Insert Size (bp)1665
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MutationL122A. Codon optimization compatible to work in Medaka and Zebrafish (other species not tested). Cryptic splicing sites removed.
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Tag
/ Fusion Protein
- oNLS (optimized nuclear localization signal) C terminal to the GFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ oDam_f_GFPoNLS was a gift from Joachim Wittbrodt (Addgene plasmid # 85819 ; http://n2t.net/addgene:85819 ; RRID:Addgene_85819) -
For your References section:
iDamIDseq and iDEAR: An improved method and computational pipeline to profile chromatin-binding proteins. Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J. Development. 2016 Oct 5. pii: dev.139261. 10.1242/dev.139261 PubMed 27707796