Skip to main content
Addgene

pCS2+ oDam_f_GFPoNLS
(Plasmid #85819)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85819 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4075
  • Total vector size (bp) 5741
  • Vector type
    Mammalian Expression, Bacterial Expression, Yeast Expression, Worm Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    oDam_f_GFPoNLS
  • Alt name
    oDamGFP
  • Species
    Dam: E. coli; GFP: Aequorea viactoria
  • Insert Size (bp)
    1665
  • Mutation
    L122A. Codon optimization compatible to work in Medaka and Zebrafish (other species not tested). Cryptic splicing sites removed.
  • Tag / Fusion Protein
    • oNLS (optimized nuclear localization signal) C terminal to the GFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ oDam_f_GFPoNLS was a gift from Joachim Wittbrodt (Addgene plasmid # 85819 ; http://n2t.net/addgene:85819 ; RRID:Addgene_85819)
  • For your References section:

    iDamIDseq and iDEAR: An improved method and computational pipeline to profile chromatin-binding proteins. Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J. Development. 2016 Oct 5. pii: dev.139261. 10.1242/dev.139261 PubMed 27707796