hFLT1 delta 754-763
(Plasmid
#86035)
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Purposehuman VEGFR1 with a 10 amino acid deletion in EC close to TM domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV6 Entry
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Backbone manufacturerOrigene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman Flt1 delta754-763 aa
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SpeciesH. sapiens (human)
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Insert Size (bp)4253
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Mutationdelta 754-763
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Entrez GeneFLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
- Promoter CMV
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Tags
/ Fusion Proteins
- FLAG (C terminal on backbone)
- HA (N terminal on backbone)
- Myc (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hFLT1 delta 754-763 was a gift from Christie Thomas (Addgene plasmid # 86035 ; http://n2t.net/addgene:86035 ; RRID:Addgene_86035) -
For your References section:
Ectodomain cleavage of FLT1 regulates receptor activation and function and is not required for its downstream intracellular cleavage. Raikwar NS, Liu KZ, Thomas CP. Exp Cell Res. 2016 May 15;344(1):103-11. doi: 10.1016/j.yexcr.2016.03.020. Epub 2016 Mar 23. 10.1016/j.yexcr.2016.03.020 PubMed 27017929
