Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17520||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5200
Vector typeMammalian Expression, Retroviral, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- TAP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
TAP tag is 6xHis-TEV-3xFLAG (note that two of the FLAG tags are not canonical--the amino acid sequence of the 3xFLAG region is: DYKDHDGDYKDHDIDYKDDDDK)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBRIT TAP was a gift from Michael Rudnicki (Addgene plasmid # 17520 ; http://n2t.net/addgene:17520 ; RRID:Addgene_17520)
For your References section:Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, Greenblatt JF, Dilworth FJ, Rudnicki MA. Nat Cell Biol. 2008 Jan . 10(1):77-84. 10.1038/ncb1671 PubMed 18066051