Addgene - pMSCV-TAP Plasmid Data

Plasmid 12570: pMSCV-TAP

Gene/insert name:
None
Fusion protein or tag:
TAP
Terminal:
N terminal on backbone
Vector backbone:
pMSCV-TAP
(Search Vector Database)
Backbone manufacturer:
Modified from Clontech's pMSCV
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
6800
5' sequencing primer:
MSCV primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
TAP was PCR'd from pBSactTAP, a gift from Dr. Matthias Wilm (Heidelberg, Germany)
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5' -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3' and right primer 5' -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3' . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clonetech) cut with BglII/EcoRI. See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al (Curr Biol. 2006 Aug 15. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12570" in your Materials and Methods section.