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pMSCV-TAP
(Plasmid #12570)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12570 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSCV-TAP
  • Backbone manufacturer
    Modified from Clontech's pMSCV
  • Backbone size (bp) 6800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • TAP (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TAP was PCR'd from pBSactTAP, a gift from Dr. Matthias Wilm (Heidelberg, Germany)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5' -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3' and right primer 5' -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3' . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See author's map for more information (Note that the NotI site is not precisely underlined in the author's map).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-TAP was a gift from David Sabatini (Addgene plasmid # 12570 ; http://n2t.net/addgene:12570 ; RRID:Addgene_12570)
  • For your References section:

    mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001 PubMed 16919458