Addgene - pMSCV-TAP Raptor Plasmid Data

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Plasmid 12587: pMSCV-TAP Raptor

Gene/insert name:
Raptor
Species:
H. sapiens (human)
Entrez Gene:
RPTOR (KOG1, Mip1, KIAA1303)
Fusion protein or tag:
TAP
Terminal:
N terminal on backbone
Vector backbone:
pMSCV
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert:
6300
Cloning site 5':
XhoI/SalI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
MSCV primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al (Curr Biol. 2006 Aug 15. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12587" in your Materials and Methods section.