pMT1121
(Plasmid #8620)

• Depositing Lab: Robert Hartley
• Publication: Hartley et al Protein Peptide Letters 1996 3:225-231

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2641
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE or HB107)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ribonuclease Sa, barstar
• Species: Streptomyces aureofaciens, Strain BMK
• Insert Size (bp): 973
• GenBank ID: U39467

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: J. Sevcik Laboratory of Molecular Biology Slovak Academy of Science, Bratislava, Slovakia

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

As Pmt1100 but with synthetic gene for ribonuclease Sa based on its amino acid sequence. Ribonuclease Sa from S. aureofaciens strain CCM 3239. 195-486: Structural gene for mature ribonuclease Sa. Genbank #U39467.