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pMT1002
(Plasmid #8621)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8621 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    barnase, barstar
  • Species
    Bacillus amyloliquefaciens
  • Insert Size (bp)
    1860
  • Mutation
    CI157 lambda repressor gene has two HindIII sitesd removed by silent mutations.
  • GenBank ID
    M14442
  • Tag / Fusion Protein
    • as pMT416 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Barnase production plasmid. The vector is the same as that of pMT416. Provides higher yields of barnase than pMT413 or pMT416. This is pTN441 [Okorokov, A.L., Hartley, R.W. and Panov, A,L, Protein Express. and Purif.
5, 547-552 (1994)
] with two HindIII sites in the CI157 gene removed by silent mutations. 900-86: The temperature sensitive repressor gene(CI157) from lambda phage, followed(3')
by its own promoter and, in the forward direction, the PR promoter for barnase. Two silent mutations in this
gene have removed HindIII sites.
901-1872:barnase & barstar genes as in pMT413 & pMT416 from BamH1 site to HindIII site. The copy number is also controlled by the lambda repressor, so that both copy number and barnase production are minimal at 30^o and high at 37^o.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT1002 was a gift from Robert Hartley (Addgene plasmid # 8621 ; http://n2t.net/addgene:8621 ; RRID:Addgene_8621)
  • For your References section:

    Barstar inhibits extracellular ribonucleases of Streptomyces and allows their production from recombinant genes. Hartley RW, Both V, Hebert EJ, Homerova D, Jucovic M, Nazarov V, Rybajlak I and Sevcik J. Protein Peptide Letters 1996 3:225-231