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Browse > Robert Hartley > Hartley et al > pMT413
Plasmid 8606: pMT413

  • barnase, barstar

  • 1071

  • Bacillus amyloliquefaciens

  • M14442 M15545

  • phoA signal sequence to barnase

  • N terminal on insert

  • pUC19
    (Search Vector Database)

  • Bacterial Expression

  • 2641

  • EcoRI

  • No

  • HindIII

  • No

  • M13F List of Sequencing Primers

  • M13R

  • Ampicillin

  • DH5alpha

  • 37

  • Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE or HB107)

  • High Copy

  • View sequences (1)

  • Robert Hartley

  • MTA

Comments: 

Barnase production (secretion)plasmid. Xba1 site is joint between barnase 3' and barstar 5' sequences. Requires an E. coli host carrying the lacI^Q gene.
Barnase and barstar genes from B. amyloliquefaciens strain H. 7-117: tac promoter and RBS.
132-194: Structural gene for propeptide phoA signal sequence.
195-628: DNA from B. amyloliquefaciens.
195-527: Structural gene for mature barnase. Genbank #M14442.
528-563: Transcription terminator.
635-1077: DNA from B. amyloliquefaciens.
763-1035: Structural gene for barstar. Genbank #X15545. Grown in Super Broth at 37oC, yield on the order of 20 mg/l is maintained from about 1 to 4 hours after growth stops.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. Hartley et al (J Mol Biol 1988 Aug 20;202(4):913-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8606" in your Materials and Methods section.

Price: $65.00

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