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pMT413
(Plasmid #8606)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8606 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE or HB107)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    barnase, barstar
  • Species
    Bacillus amyloliquefaciens
  • Insert Size (bp)
    1071
  • GenBank ID
    M14442 M15545
  • Tag / Fusion Protein
    • phoA signal sequence to barnase (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Barnase production (secretion)plasmid. Xba1 site is joint between barnase 3' and barstar 5' sequences. Requires an E. coli host carrying the lacI^Q gene.
Barnase and barstar genes from B. amyloliquefaciens strain H. 7-117: tac promoter and RBS.
132-194: Structural gene for propeptide phoA signal sequence.
195-628: DNA from B. amyloliquefaciens.
195-527: Structural gene for mature barnase. Genbank #M14442.
528-563: Transcription terminator.
635-1077: DNA from B. amyloliquefaciens.
763-1035: Structural gene for barstar. Genbank #X15545. Grown in Super Broth at 37oC, yield on the order of 20 mg/l is maintained from about 1 to 4 hours after growth stops.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT413 was a gift from Robert Hartley (Addgene plasmid # 8606 ; http://n2t.net/addgene:8606 ; RRID:Addgene_8606)
  • For your References section:

    Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. Hartley RW. J Mol Biol 1988 Aug 20;202(4):913-5. 10.1016/0022-2836(88)90568-2 PubMed 3050134