|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86304||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namegRNA against MBD1_iso1
SpeciesH. sapiens (human)
Entrez GeneMBD1 (a.k.a. CXXC3, PCM1, RFT)
- Cloning method Gibson Cloning
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PX458_MBD1_iso1_2 was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 86304 ; http://n2t.net/addgene:86304 ; RRID:Addgene_86304)
For your References section:CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. Genome Res. 2015 Sep 9. 10.1101/gr.193540.115 PubMed 26355004