pCx55.5-TrpR
(Plasmid
#86373)
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Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDestTol2pA
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Vector typezebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTrpR
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Alt nameTryptophan repressor
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SpeciesE. coli
- Promoter cx55.5
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer mCherry-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCx55.5-TrpR was a gift from Rachel Wong (Addgene plasmid # 86373 ; http://n2t.net/addgene:86373 ; RRID:Addgene_86373) -
For your References section:
Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo. Yoshimatsu T, D'Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, Raible DW, Wong RO. Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590. 10.1038/ncomms10590 PubMed 26838932
