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RN3P-KDM5B-FLAG
(Plasmid #86398)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86398 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RN3P
  • Backbone manufacturer
    Derivative of pBluescript RN3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDM5B
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Kdm5b (a.k.a. 2010009J12Rik, 2210016I17Rik, AW556288, D1Ertd202, D1Ertd202e, Jari, Jarid1b, PLU, PLU-1, PUT1, Pl, Plu1, RBBP2H1A, Rb-B, Rb-Bp2, mKIAA4034)
  • Promoter Lac
  • Tag / Fusion Protein
    • 3x-FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer XBG-R (GACTCCATTCGGGTGTTC)
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RN3P-KDM5B-FLAG was a gift from Wei Xie (Addgene plasmid # 86398 ; http://n2t.net/addgene:86398 ; RRID:Addgene_86398)
  • For your References section:

    Allelic reprogramming of the histone modification H3K4me3 in early mammalian development. Zhang B, Zheng H, Huang B, Li W, Xiang Y, Peng X, Ming J, Wu X, Zhang Y, Xu Q, Liu W, Kou X, Zhao Y, He W, Li C, Chen B, Li Y, Wang Q, Ma J, Yin Q, Kee K, Meng A, Gao S, Xu F, Na J, Xie W. Nature. 2016 Sep 14;537(7621):553-557. doi: 10.1038/nature19361. 10.1038/nature19361 PubMed 27626382