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pET14-GFP15_Encap
(Plasmid #86407)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86407 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6600
  • Modifications to backbone
    BsaI and BsmBI recognition sites were removed
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sfGFP with a 15aa CLP tag
  • Species
    Thermatoga maritima
  • Insert Size (bp)
    800
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Encapsulin
  • Species
    Thermatoga maritima
  • Insert Size (bp)
    800
  • Promoter T7
  • Tag / Fusion Protein
    • 6x Histidine (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET14-GFP15_Encap was a gift from David Savage (Addgene plasmid # 86407 ; http://n2t.net/addgene:86407 ; RRID:Addgene_86407)
  • For your References section:

    Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, East-Seletsky A, Williams ER, Savage DF. Biochemistry. 2016 Jun 21;55(24):3461-8. doi: 10.1021/acs.biochem.6b00294. Epub 2016 Jun 7. 10.1021/acs.biochem.6b00294 PubMed 27224728