p3868 HA-E6-AP
(Plasmid #8648)

• Depositing Lab: Peter Howley
• Publication: Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Backbone

• Vector backbone: pCMV4
• Backbone size w/o insert (bp): 4874
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E6-AP
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Entrez Gene: UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Addgene Notes:
  • Poor protein expression
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).