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p3868 HA-E6-AP
(Plasmid #8648)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8648 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E6-AP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See author's map. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3868 HA-E6-AP was a gift from Peter Howley (Addgene plasmid # 8648)
  • For your References section:

    The role of E6AP in the regulation of p53 protein levels in human papillomavirus (HPV)-positive and HPV-negative cells. Talis AL, Huibregtse JM, Howley PM. J Biol Chem 1998 Mar 13;273(11):6439-45. 10.1074/jbc.273.11.6439 PubMed 9497376