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CIAR_pcDNA5/FRT/TO
(Plasmid #86502)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86502 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 7339
  • Vector type
    Mammalian Expression ; FRT
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIAR
  • Alt name
    S.17.3
  • Alt name
    Chemically Inducible Activator of RAS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2202
  • Mutation
    T968L in SOScat
  • Entrez Gene
    SOS1 (a.k.a. GF1, GGF1, GINGF, HGF, NS4, SOS-1)
  • Promoter CMV (tet operator)

Cloning Information

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CIAR_pcDNA5/FRT/TO was a gift from Dustin Maly (Addgene plasmid # 86502 ; http://n2t.net/addgene:86502 ; RRID:Addgene_86502)
  • For your References section:

    A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics. Rose JC, Huang PS, Camp ND, Ye J, Leidal AM, Goreshnik I, Trevillian BM, Dickinson MS, Cunningham-Bryant D, Debnath J, Baker D, Wolf-Yadlin A, Maly DJ. Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244. 10.1038/nchembio.2244 PubMed 27870838