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A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics.
Rose JC, Huang PS, Camp ND, Ye J, Leidal AM, Goreshnik I, Trevillian BM, Dickinson MS, Cunningham-Bryant D, Debnath J, Baker D, Wolf-Yadlin A, Maly DJ
Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86502CIAR_pcDNA5/FRT/TOExpresses CIAR in mammalian cells. Used to generate Flp-In stables.
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86503GFP-P2A-CIAR_pLenti_CMV_PuroLenti plasmid for generating CIAR expressing stable cell lines
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86504xCIAR_pcDNA5/FRT/TOExpresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.
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86505CRAF_S259A_pcDNA3.1Expresses CRAF_S259A in mammalian cells
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86506CRAF_S29A_pcDNA3.1Expresses CRAF_S29A in mammalian cells [Edit]
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