GFP-P2A-CIAR_pLenti_CMV_Puro
(Plasmid #86503)

• Purpose: Lenti plasmid for generating CIAR expressing stable cell lines
• Depositing Lab: Dustin Maly
• Publication: Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038/nchembio.2244.

Backbone

• Vector backbone: pLenti CMV GFP Puro (Addgene 17448)
• Backbone manufacturer: Eric Campeau
• Backbone size w/o insert (bp): 7888
• Total vector size (bp): 10885
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-P2A-CIAR
• Species: H. sapiens (human)
• Insert Size (bp): 2997
• Mutation: T968L in SOScat
• Entrez Gene: SOS1 (a.k.a. GF1, GGF1, GINGF, HGF, NS4, SOS-1)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_F

Resource Information

• Supplemental Documents:
  • GFP_P2A_S.17.3_pLenti_CMV_Puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry