Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8667||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerKuo et al, Mol Cell Biol. 1983 October; 3(10): 1730
Vector typeYeast Expression ; Centromeric
Growth in Bacteria
Growth instructionsDon't know
SpeciesS. cerevisiae (budding yeast)
Entrez GeneTUB1 (a.k.a. YML085C)
- Cloning method Restriction Enzyme
- 5′ cloning site See comment (unknown if destroyed)
- 3′ cloning site See comment (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The TUB1 centromere plasmid was constructed by ligating a TUB1 fragment from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI fragment of YCp50. For more information on YCp50, including map, search for YCp50 in Addgene's vector db.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRB326 was a gift from David Botstein (Addgene plasmid # 8667 ; http://n2t.net/addgene:8667 ; RRID:Addgene_8667)
For your References section:Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Schatz PJ, Solomon F, Botstein D. Mol Cell Biol 1986 Nov;6(11):3722-33. PubMed 3540600
Map uploaded by the depositor.