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pRB2960
(Plasmid #8677)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8677 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    Don't know
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP::TUB1 under GAL1 promoter
  • Alt name
    TUB1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    TUB1 (a.k.a. YML085C)
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See comment (unknown if destroyed)
  • 3′ cloning site See comment (unknown if destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TUB1 wildtype DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB2960 was a gift from David Botstein (Addgene plasmid # 8677 ; http://n2t.net/addgene:8677 ; RRID:Addgene_8677)
  • For your References section:

    Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast. Anders KR, Botstein D. Mol Biol Cell 2001 Dec;12(12):3973-86. 10.1091/mbc.12.12.3973 PubMed 11739794