Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8677||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Growth instructionsDon't know
Gene/Insert nameGFP::TUB1 under GAL1 promoter
SpeciesS. cerevisiae (budding yeast)
Entrez GeneTUB1 (a.k.a. YML085C)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site See comment (unknown if destroyed)
- 3′ cloning site See comment (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
TUB1 wildtype DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRB2960 was a gift from David Botstein (Addgene plasmid # 8677 ; http://n2t.net/addgene:8677 ; RRID:Addgene_8677)
For your References section:Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast. Anders KR, Botstein D. Mol Biol Cell 2001 Dec;12(12):3973-86. 10.1091/mbc.12.12.3973 PubMed 11739794