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pBOB-EF1-FastFUCCI-Puro
(Plasmid #86849)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86849 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBOB
  • Backbone manufacturer
    Verma lab (Salk Institute)
  • Backbone size w/o insert (bp) 7800
  • Total vector size (bp) 12350
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mKO2-hCDT1(30-120)
  • Alt name
    Fucci-G1 phase
  • Species
    Synthetic
  • Insert Size (bp)
    978
  • GenBank ID
    AB370332.1
  • Promoter EF1

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer TGATGTAATTCTCCTTGG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mAG-hGEM(1-110)
  • Alt name
    Fucci-S-G2-M phase
  • Species
    Synthetic
  • Insert Size (bp)
    1038
  • GenBank ID
    AB370333
  • Promoter EF1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer AGAAGAGCACACCTGCCG
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    pac (puromycin N-acetyl-transferase)
  • Alt name
    puromycin resistance gene
  • Species
    S. alboniger
  • Insert Size (bp)
    600
  • GenBank ID
    M25346.1
  • Promoter PGK

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site none (destroyed during cloning)
  • 5′ sequencing primer ggcattctgcacgcttca
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBOB-EF1-FastFUCCI-Puro was a gift from Kevin Brindle & Duncan Jodrell (Addgene plasmid # 86849 ; http://n2t.net/addgene:86849 ; RRID:Addgene_86849)
  • For your References section:

    Quantitative FastFUCCI assay defines cell cycle dynamics at single-cell level. Koh SB, Mascalchi P, Rodriguez E, Lin Y, Jodrell DI, Richards FM, Lyons SK. J Cell Sci. 2016 Nov 25. pii: jcs.195164. 10.1242/jcs.195164 PubMed 27888217