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(Plasmid #87121)


Item Catalog # Description Quantity Price (USD)
Plasmid 87121 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5358
  • Total vector size (bp) 6358
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Renilla Luciferase
  • Alt name
  • Species
    Sea pansy, Renilla reniformis
  • Insert Size (bp)
  • Mutation
    A55T, C124A, S130A, K136R, A143M, M185V, M253L, and S287L
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-RLuc8 was a gift from Sanjiv Sam Gambhir (Addgene plasmid # 87121 ; ; RRID:Addgene_87121)
  • For your References section:

    Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Loening AM, Fenn TD, Wu AM, Gambhir SS. Protein Eng Des Sel. 2006 Sep;19(9):391-400. Epub 2006 Jul 20. 10.1093/protein/gzl023 PubMed 16857694