Addgene - pKT0239 Plasmid Data

Plasmid 8738: pKT0239

Gene/insert name:
yECitrine
Alt name:
Codon-optimized GFP
Fusion protein or tag:
Codon optimized linker
Terminal:
N terminal on backbone
Fusion protein or tag:
3HA
Terminal:
C terminal on backbone
Mutation:
yEGFP with S65G, V68L, Q69M, S72A, T203Y
Vector backbone:
pFA6a-link
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
4901
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers, other:
S. pombe HIS5
Sequence: View sequences (2)
Principal Investigator:
Kurt Thorn
Terms and Licenses:
MTA

Comments:

Full name of this plasmid is pFA6a-link-yECitrine-3HA-SpHIS5. yECitrine is created by mutagenesis from a codon-optimized green
fluorescent protein - yEGFP1 (Cormack et al. 1997). This yEGFP
variant is cloned into pDH5, replacing YFP. An codon-optimized linker
was added into the yEGFP variant to improve expression level. The
selectable marker, Sz. pombe HIS5 (SpHIS5), complements S. cerevisiae
HIS3.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff et al (Yeast 2004 Jun;21(8):661-70. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8738" in your Materials and Methods section.