Addgene - pB18cmBFPAzurite Plasmid Data

Plasmid 14034: pB18cmBFPAzurite

Gene/insert name:
Azurite
Fusion protein or tag:
6xHis
Terminal:
C terminal on insert
Vector backbone:
pBAD18-Cm
(Search Vector Database)
Vector type:
Bacterial Expression ; Yeast/Bacteria optimized codons
Backbone size w/o insert (bp):
6026
Cloning site 5':
SfiI
Site destroyed during cloning:
No
Cloning site 3':
SfiI
Site destroyed during cloning:
No
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Patrick Daugherty
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Mena et al (Nat Biotechnol. 2006 Dec . 24(12):1569-71. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14034" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

14030: pCyPet-His

14033: pCEP4CyPet-MAMM

14035: pB18cmBFP1A5

14031: pYPet-His

14032: pCEP4YPet-MAMM