Addgene - pYPet-His Plasmid Data

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14031: pYPet-His
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Plasmid 14031: pYPet-His

Gene/insert name:
YPet
Fusion protein or tag:
6xHis
Terminal:
C terminal on insert
Vector backbone:
pBAD33
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert:
5352
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Patrick Daugherty
Terms and Licenses:
MTA

Comments:

SfiI sites were added before and after gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Evolutionary optimization of fluorescent proteins for intracellular FRET. Nguyen et al (Nat Biotechnol. 2005 Mar . 23(3):355-60. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14031" in your Materials and Methods section.