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(Plasmid #87451)


Item Catalog # Description Quantity Price (USD)
Plasmid 87451 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    FKBP and rapamycin binding domain only
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
  • Promoter CMV
  • Tags / Fusion Proteins
    • ras fragment (C terminal on insert)
    • CFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

Depositor Comments

ras fragment (60 bp)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-CFP-ras was a gift from Xiaodong Liu (Addgene plasmid # 87451 ; ; RRID:Addgene_87451)
  • For your References section:

    Cooperative and acute inhibitionby multiple C-terminal motifs of L-type Ca2+ channels. Liu N, Yang Y, Ge L, Liu M, Colecraft HM, Liu X. Elife. 2017 Jan 6;6. pii: e21989. doi: 10.7554/eLife.21989. 10.7554/eLife.21989 PubMed 28059704