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pcDNA-I-PhoCl-HCVp
(Plasmid #87697)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87697 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • EBFP (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Please note that there's a M to I discrepancy between the reference sequence and Addgene's quality control sequence. The change does not affect EBFP or PhoCI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-I-PhoCl-HCVp was a gift from Robert Campbell (Addgene plasmid # 87697)
  • For your References section:

    Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123