PurposeExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||87697||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert nameI-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
- Promoter CMV
/ Fusion Protein
- EBFP (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please note that there's a M to I discrepancy between the reference sequence and Addgene's quality control sequence. The change does not affect EBFP or PhoCI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-I-PhoCl-HCVp was a gift from Robert Campbell (Addgene plasmid # 87697 ; http://n2t.net/addgene:87697 ; RRID:Addgene_87697)
For your References section:Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123