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Optogenetic control with a photocleavable protein, PhoCl.
Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE
Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87688pBAD/HisB-PhoClExpress PhoCl in E. coli
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87689pBAD/HisB-PhoCl-MBPExpress PhoCl-MBP in E.coli
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87690pcDNA-NES-PhoCl-mCherryExpress NES-PhoCl-mCherry in mammalian cells
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87691pcDNA-NLS-PhoCl-mCherryExpress NLS-PhoCl-mCherry in mammalian cells
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87692pcDNA-PhoCl-mCherry-mycExpress PhoCl-mCherry with C-term myc tag in mammalian cells
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87693pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells
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87694pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells
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87695pUAS-mCherry-NLSGal4VP16 driven expression of mCherry-NLS in mammalian cells
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87696pUAS-NanoLucGal4VP16 driven expression of NanoLuc
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87697pcDNA-I-PhoCl-HCVpExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cells
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87698pcDNA-PANX1(HCVp)Express HCV protease-activatible pannexin-1 in mammalian cells
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87699pcDNA-mCherry-4A/4B-CAAXExpress mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells
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