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pPTluc
(Plasmid #87789)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87789 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPTGAL
  • Backbone manufacturer
    Daniel F. Eberl lab, PMID 12324963
  • Backbone size (bp) 9890
  • Modifications to backbone
    replace a 3193bp fragment containing Gal4 flanked by PstI sites with a 1955bp HindIII-BamHI fragment containing the luciferase gene from pGL3-Basic (Promega).
  • Vector type
    Insect Expression
  • Promoter minimal promoter
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPTluc was a gift from Herman Wijnen (Addgene plasmid # 87789 ; http://n2t.net/addgene:87789 ; RRID:Addgene_87789)
  • For your References section:

    A new promoter element associated with daily time keeping in Drosophila. Sharp B, Paquet E, Naef F, Bafna A, Wijnen H. Nucleic Acids Res. 2017 Apr 12. doi: 10.1093/nar/gkx268. 10.1093/nar/gkx268 PubMed 28407113