pPTluc
(Plasmid
#87789)
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Purpose(Empty Backbone) Luciferase reporter for testing enhancer fragments in Drosophila
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepPTGAL
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Backbone manufacturerDaniel F. Eberl lab, PMID 12324963
- Backbone size (bp) 9890
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Modifications to backbonereplace a 3193bp fragment containing Gal4 flanked by PstI sites with a 1955bp HindIII-BamHI fragment containing the luciferase gene from pGL3-Basic (Promega).
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Vector typeInsect Expression
- Promoter minimal promoter
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCasper-F
- 3′ sequencing primer lucNRev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPTluc was a gift from Herman Wijnen (Addgene plasmid # 87789 ; http://n2t.net/addgene:87789 ; RRID:Addgene_87789) -
For your References section:
A new promoter element associated with daily time keeping in Drosophila. Sharp B, Paquet E, Naef F, Bafna A, Wijnen H. Nucleic Acids Res. 2017 Apr 12. doi: 10.1093/nar/gkx268. 10.1093/nar/gkx268 PubMed 28407113
