|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8845||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 9500
Vector typeInsect Expression
/ Fusion Protein
- biotin acceptor peptide (N terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
This baculovirus transfer vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused to its N-terminus in Spodoptera frugiperda cells. pDW464 also overproduces E. coli biotin holoenzyme synthetase (BirA), under polyhedron promoter control, so that BAP-tagged proteins can be biotinylated in Spodoptera frugiperda cells. pDW464 is designed to use in conjunction with the Bac-to-Bac system (Invitrogen) for constructing recombinant baculoviruses.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDW464 was a gift from David Waugh (Addgene plasmid # 8845 ; http://n2t.net/addgene:8845 ; RRID:Addgene_8845)
For your References section:Site-specific, enzymatic biotinylation of recombinant proteins in Spodoptera frugiperda cells using biotin acceptor peptides. Duffy S, Tsao KL, Waugh DS. Anal Biochem. 1998 Sep 10. 262(2):122-8. 10.1006/abio.1998.2770 PubMed 9750126