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Plasmid 8845: pDW464
  • None

  • p2Bac
    (Search Vector Database)

  • Invitrogen

  • Insect Expression

  • 9500

  • biotin acceptor peptide

  • N terminal on backbone

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • David Waugh

  • UBMTA

Comments: 

This baculovirus transfer vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused to its N-terminus in Spodoptera frugiperda cells. pDW464 also overproduces E. coli biotin holoenzyme synthetase (BirA), under polyhedron promoter control, so that BAP-tagged proteins can be biotinylated in Spodoptera frugiperda cells. pDW464 is designed to use in conjunction with the Bac-to-Bac system (Invitrogen) for constructing recombinant baculoviruses.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Site-specific, enzymatic biotinylation of recombinant proteins in Spodoptera frugiperda cells using biotin acceptor peptides. Duffy et al (Anal Biochem. 1998 Sep 10. 262(2):122-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8845" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only