Addgene - pDW464 Plasmid Data

Plasmid 8845: pDW464

Gene/insert name:
biotin acceptor peptide, BirA
Alt name:
BAP
Insert size:
1200
Species:
E. coli
Fusion protein or tag:
biotin acceptor peptide
Terminal:
N terminal on insert
Vector backbone:
p2Bac
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression
Backbone size w/o insert (bp):
9500
Cloning site 5':
NcoI
Site destroyed during cloning:
No
Cloning site 3':
SacI
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Waugh
Terms and Licenses:
MTA

Comments:

This baculovirus transfer vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused to its N-terminus in Spodoptera frugiperda cells. pDW464 also overproduces E. coli biotin holoenzyme synthetase (BirA), under polyhedron promoter control, so that BAP-tagged proteins can be biotinylated in Spodoptera frugiperda cells. pDW464 is designed to use in conjunction with the Bac-to-Bac system (Invitrogen) for constructing recombinant baculoviruses.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Site-specific, enzymatic biotinylation of recombinant proteins in Spodoptera frugiperda cells using biotin acceptor peptides. Duffy et al (Anal Biochem. 1998 Sep 10. 262(2):122-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8845" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

8846: pDW465

11086: pDEST-periHisMBP

11085: pDEST-HisMBP

8835: pRK1043

8832: pRK1035