pDW464
(Plasmid #8845)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p2Bac
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 9500
  • Vector type
    Insect Expression
  • Tag / Fusion Protein
    • biotin acceptor peptide (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses

Depositor Comments

This baculovirus transfer vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused to its N-terminus in Spodoptera frugiperda cells. pDW464 also overproduces E. coli biotin holoenzyme synthetase (BirA), under polyhedron promoter control, so that BAP-tagged proteins can be biotinylated in Spodoptera frugiperda cells. pDW464 is designed to use in conjunction with the Bac-to-Bac system (Invitrogen) for constructing recombinant baculoviruses.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDW464 was a gift from David Waugh (Addgene plasmid # 8845)
  • For your References section:

    Site-specific, enzymatic biotinylation of recombinant proteins in Spodoptera frugiperda cells using biotin acceptor peptides. Duffy S, Tsao KL, Waugh DS. Anal Biochem. 1998 Sep 10. 262(2):122-8. 10.1006/abio.1998.2770 PubMed 9750126