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pFlpStop-attB-UAS-2.1-tdTom
(Plasmid #88910)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 88910 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS-KS-attB1-2
  • Backbone manufacturer
    Gene Disruption Project, see Venken et al., 2011, Nat. Methods 8(9): 737-743
  • Backbone size w/o insert (bp) 4752
  • Total vector size (bp) 7630
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlpStop
  • Species
    Synthetic
  • Insert Size (bp)
    2878
  • Promoter 5XUAS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GGAACTTCTCTTTGCACCATTT
  • 3′ sequencing primer CCCAGGCTTTACACTTTATG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFlpStop-attB-UAS-2.1-tdTom was a gift from Tom Clandinin (Addgene plasmid # 88910 ; http://n2t.net/addgene:88910 ; RRID:Addgene_88910)
  • For your References section:

    FlpStop, a tool for conditional gene control in Drosophila. Fisher YE, Yang HH, Isaacman-Beck J, Xie M, Gohl DM, Clandinin TR. Elife. 2017 Feb 17;6. pii: e22279. doi: 10.7554/eLife.22279. 10.7554/eLife.22279 PubMed 28211790