Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8908||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5200
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
Yeast GST expression plasmid, XbaI oligo inserted into pGEX-2TB to convert XbaI site to BglII; 0.7-kb BglII-BamHI fragment of pGEX-2TB inserted into BamHI site of pPS293, GAL1 promoter, 2 micron origin. See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPS892 was a gift from Pamela Silver (Addgene plasmid # 8908)
For your References section:Dynamic localization of the nuclear import receptor and its interactions with transport factors. Koepp DM, Wong DH, Corbett AH, Silver PA. J Cell Biol. 1996 Jun . 133(6):1163-76. 10.1083/jcb.133.6.1163 PubMed 8682856
Map uploaded by the depositor.