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pBS/U6 radixin siRNA
(Plasmid #8947)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    radixin siRNA
  • Alt name
    radixin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    50
  • Entrez Gene
    RDX (a.k.a. DFNB24)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

siRNA targeting radixin. Sense strand of the hairpin identical to a 22 nucleotide region in the target gene (radixin [929-949]).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS/U6 radixin siRNA was a gift from Philip Hinds (Addgene plasmid # 8947)
  • For your References section:

    Increased ezrin expression and activation by CDK5 coincident with acquisition of the senescent phenotype. Yang HS, Hinds PW. Mol Cell. 2003 May . 11(5):1163-76. 10.1016/S1097-2765(03)00135-7 PubMed 12769842