Addgene - Rc/CMV cyclin D1 Plasmid Data

Plasmid 8962: Rc/CMV cyclin D1

Gene/insert name:
cyclin D1
Insert size:
1100
Species:
H. sapiens (human)
Entrez Gene:
CCND1 (BCL1, D11S287E, PRAD1, U21B31)
Vector backbone:
pRc/CMV
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5542
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

See author's map.

Please note that some user sequence suggests that there may be some silent mutations present in 3' end of the cyclin D1 gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Hinds et al (Cell. 1992 Sep 18. 70(6):993-1006. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8962" in your Materials and Methods section.