Rc/CMV cyclin D1
(Plasmid #8962)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRc/CMV
  • Backbone size w/o insert (bp) 5542
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    cyclin D1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • Entrez Gene
    CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

See author's map.

Please note that some user sequence suggests that there may be some silent mutations present in 3' end of the cyclin D1 gene.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rc/CMV cyclin D1 was a gift from Bob Weinberg (Addgene plasmid # 8962)
  • For your References section:

    Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Hinds PW, Mittnacht S, Dulic V, Arnold A, Reed SI, Weinberg RA. Cell. 1992 Sep 18. 70(6):993-1006. 10.1016/0092-8674(92)90249-C PubMed 1388095