Rc/CMV cyclin D1
- Backbone size w/o insert (bp) 5542
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert namecyclin D1
SpeciesH. sapiens (human)
Insert Size (bp)1100
Entrez GeneCCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
See author's map.
Please note that some user sequence suggests that there may be some silent mutations present in 3' end of the cyclin D1 gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Rc/CMV cyclin D1 was a gift from Bob Weinberg (Addgene plasmid # 8962)
For your References section:Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Hinds PW, Mittnacht S, Dulic V, Arnold A, Reed SI, Weinberg RA. Cell. 1992 Sep 18. 70(6):993-1006. 10.1016/0092-8674(92)90249-C PubMed 1388095
Map uploaded by the depositor.