Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89873||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7466
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)3096
MutationR173H in TdTomato (Please see depositor comments below)
- Promoter Human synapsin
/ Fusion Protein
- TdTomato (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Please note that mutation R173H in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA was a gift from Hyungbae Kwon (Addgene plasmid # 89873 ; http://n2t.net/addgene:89873 ; RRID:Addgene_89873)
For your References section:Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Lee D, Creed M, Jung K, Stefanelli T, Wendler DJ, Oh WC, Mignocchi NL, Luscher C, Kwon HB. Nat Methods. 2017 May;14(5):495-503. doi: 10.1038/nmeth.4234. Epub 2017 Apr 3. 10.1038/nmeth.4234 PubMed 28369042