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Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain.
Lee D, Creed M, Jung K, Stefanelli T, Wendler DJ, Oh WC, Mignocchi NL, Luscher C, Kwon HB
Nat Methods. 2017 May;14(5):495-503. doi: 10.1038/nmeth.4234. Epub 2017 Apr 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
89869pCMV-bArrestin2-TevC-P2A-TdTomatoexpresses iTango bArrestin2-TevC component and TdTomato bicistronically
89870pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16expresses DRD2-iTango component in mammalian cells
89871pTRE-EGFPEGFP reporter vector for iTango system
89872pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16expresses CB1R-iTango component in mammalian cells
89873pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpAexpresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production
89874pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpAexpresses DRD2-iTango component in mammalian cells for AAV production
89875pAAV-TRE-EGFPEGFP reporter vector of iTango system for AAV production
89876pAAV-TRE-FLEX-eNpHR-EYFPTo generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter
89877pCMV-NES-CRY2PHR-TevCexpresses NES-CRY2PHR-TevC of BLITz system
89878pCMV-TM-CIBN-BLITz1-TetR-VP16expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

Antibodies from Article