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pRK7-FLAG-TSC1
(Plasmid #8995)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8995 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSC1
  • Alt name
    hamartin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Entrez Gene
    TSC1 (a.k.a. LAM, TSC)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene sequencing results find amino acid substitutions R768H and I812M compared to hamartin reference sequence NP_000359.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK7-FLAG-TSC1 was a gift from John Blenis (Addgene plasmid # 8995 ; http://n2t.net/addgene:8995 ; RRID:Addgene_8995)
  • For your References section:

    Tuberous sclerosis complex-1 and -2 gene products function together to inhibit mammalian target of rapamycin (mTOR)-mediated downstream signaling. Tee AR, Fingar DC, Manning BD, Kwiatkowski DJ, Cantley LC, Blenis J. Proc Natl Acad Sci U S A. 2002 Oct 15. 99(21):13571-6. 10.1073/pnas.202476899 PubMed 12271141