Addgene - 1099 pcDNA GFP FKHR AAA Plasmid Data

Plasmid 9023: 1099 pcDNA GFP FKHR AAA

Gene/insert name:
FKHR AAA
Alt name:
FKHR
Alt name:
FOXO1A
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
FOXO1 (FKH1, FKHR, FOXO1A)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Mutation:
T24A S256A S319A
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6046
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Comments:

AAA mutant cannot be phosphorylated by Akt

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Nakamura et al (Mol Cell Biol. 2000 Dec . 20(23):8969-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9023" in your Materials and Methods section.