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Plasmid 9025: 1119 pBabe puroL HA FKHR AAA
  • FKHR AAA

  • FKHR

  • FOXO1A

  • 2000

  • H. sapiens (human)

  • FOXO1 (FKH1, FKHR, FOXO1A)

  • HA

  • N terminal on backbone

  • T24A S256A S319A

  • pBabe puro L
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5200

  • BamHI

  • No

  • XhoI

  • No

  • pBabe5 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • View map

  • William Sellers

  • UBMTA

Comments: 

Oligonucleotides 5'-GCGCGGATCCATGGCCGAGGCGCCTCAGGTG-3' and 5'-CGCGCTCGAGGAATTCTCAGCCTGACACCCAGCTATG-3' were used to PCR amplify the FKHR cDNA. The PCR products, restricted with BamHI and XhoI, were ligated to similarly restricted pBABE-puroL to give pBABE-puroL-FKHR. AAA mutant cannot be phosphorylated by Akt.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Nakamura et al (Mol Cell Biol. 2000 Dec . 20(23):8969-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9025" in your Materials and Methods section.