Addgene - 1119 pBabe puroL HA FKHR AAA Plasmid Data

Plasmid 9025: 1119 pBabe puroL HA FKHR AAA

Gene/insert name:
FKHR AAA
Alt name:
FKHR
Alt name:
FOXO1A
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
FOXO1 (FKH1, FKHR, FOXO1A)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Mutation:
T24A S256A S319A
Vector backbone:
pBabe puro L
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5200
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pBabe5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Comments:

Oligonucleotides 5'-GCGCGGATCCATGGCCGAGGCGCCTCAGGTG-3' and 5'-CGCGCTCGAGGAATTCTCAGCCTGACACCCAGCTATG-3' were used to PCR amplify the FKHR cDNA. The PCR products, restricted with BamHI and XhoI, were ligated to similarly restricted pBABE-puroL to give pBABE-puroL-FKHR. AAA mutant cannot be phosphorylated by Akt.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Nakamura et al (Mol Cell Biol. 2000 Dec . 20(23):8969-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9025" in your Materials and Methods section.