pmVirD2
(Plasmid #90314)

• Purpose: mVirD2 expression plasmid
• Depositing Lab: Bjorn Hogberg
• Publication: Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e160. doi: 10.1093/nar/gkx707.

Backbone

• Vector backbone: pSNAP-tag® (T7)-2
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 6087
• Total vector size (bp): 6720
• Modifications to backbone: insertion of a T5 lac promoter upstream the gene
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: A. tumefaciens VirD2 from aa 1 to 204
• Species: A. tumefaciens
• Insert Size (bp): 633
• GenBank ID: M17989.1
• Promoter: T7/T5 IPTG inducible
• Tag / Fusion Protein:
  • His6x tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: His tag universal primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry