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pLEX_305-N-dTAG
(Plasmid #91797)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 91797 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 91797-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLEX_305
  • Backbone manufacturer
    David Root Lab (addgene #41390)
  • Backbone size (bp) 9621
  • Modifications to backbone
    FKBP F36V tag (dTAG) added to the 5' end of the gateway cassette.
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter hPGK
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • FKBP F36V (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB resistant bacteria. 30C for liquid culture and for plates.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TGTTCCGCATTCTGCAAGCCTC
  • 3′ sequencing primer ACAAAGGCATTAAAGCAGCGTATCC
  • (Common Sequencing Primers)

Resource Information

Information for Cloning Grade DNA (Catalog # 91797-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX_305-N-dTAG was a gift from James Bradner & Behnam Nabet (Addgene plasmid # 91797 ; http://n2t.net/addgene:91797 ; RRID:Addgene_91797)
  • For your References section:

    The dTAG system for immediate and target-specific protein degradation. Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, Yang A, Leggett AL, Erb MA, Lawlor MA, Souza A, Scott TG, Vittori S, Perry JA, Qi J, Winter GE, Wong KK, Gray NS & Bradner JE.. Nature Chemical Biology (2018) 10.1038/s41589-018-0021-8