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The dTAG system for immediate and target-specific protein degradation
Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, Yang A, Leggett AL, Erb MA, Lawlor MA, Souza A, Scott TG, Vittori S, Perry JA, Qi J, Winter GE, Wong KK, Gray NS & Bradner JE.
Nature Chemical Biology (2018)
Article

Plasmids from Article

ID Plasmid Purpose  
91792pCRIS-PITChv2-BSD-dTAG (BRD4)PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.
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91793pCRIS-PITChv2-Puro-dTAG (BRD4)PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.
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91794pX330A-nBRD4/PITChPITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4
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91795pCRIS-PITChv2-dTAG-BSD (BRD4)PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.
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91796pCRIS-PITChv2-dTAG-Puro (BRD4)PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.
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91797pLEX_305-N-dTAGLentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system
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91798pLEX_305-C-dTAGLentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system
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